High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo
High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo Detail:
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. Product Information
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis.
2. Product Parameter
Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)
0.1-1.0EU/ml (assay time about 16 minutes)
3. Product Features and Application
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Note:
Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.
|
Catalog No. |
Description |
Kit Contents |
Sensitivity EU/ml |
|
EC80545 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling), 80 Tests/Kit |
5 Lyophilized Amebocyte Lysate, 1.7ml/vial; 4 Water for BET, 50ml/vial; 5 CSE; 5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml /vial; 5 Diazo Reagent 3, 10ml/vial; |
0.1 – 1 EU/ml |
|
EC80545S |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Does the End point endotoxin test kit need sophisticated microplate reader?
Bioendo EC80545 and EC80545S, can read by regular spectrophotometer.
Product detail pictures:
Related Product Guide:
We often stay with the principle "Quality Very first, Prestige Supreme". We have been fully committed to supplying our consumers with competitively priced high-quality goods, prompt delivery and skilled provider for High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Azerbaijan, European, Niger, Immediate and specialist after-sale service supplied by our consultant group has happy our buyers. Detailed Info and parameters from the merchandise will probably be sent to you for any thorough acknowledge. Free samples may be delivered and company check out to our corporation. n Morocco for negotiation is constantly welcome. Hope to get inquiries type you and construct a long-term co-operation partnership.
By Cornelia from Paraguay - 2018.07.27 12:26
The company's products very well, we have purchased and cooperated many times, fair price and assured quality, in short, this is a trustworthy company!
By Katherine from Cape Town - 2018.07.27 12:26
Leave Your Messages
Related products
-
Factory Cheap Hot limulus amebocyte lysate kit ...
Endotoxin-specific Lyophilized Amebocyte Lysate 1. Product Information Lyophilized Amebocyte Lysate extracted from horseshoe crab contains factor C pathway which reacts to endotoxin and factor G pathway which reacts to (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate contains ß-Glucan inhibitor, which will avoid false positive, result happening between factor C pathway and (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate is especially suitable for samples ...
-
High Quality Kinetic Chromogenic Endotoxin test...
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) 1. Product Information Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum abso...
-
Wholesale Pyrogen free tip - Single-Channel Me...
Single-Channel Mechanical Pipettes (Semi-sterile) 1. Product Information The Single-Channel Mechanical Pipettes (Semi-sterile) is adjustable, which could be used to dispense liquid precisely. Measuring volume of our Single-Channel Mechanical Pipettes (Semi-sterile) ranges from 0.1μL to 5mL. Products are produced based on ISO8655/DIN12650. It could be widely employed on endotoxin detection, etc.. 2. Product Features - Light weight, economic - The pipettes cover volume range from 0.1μL to 5...
-
Factory Free sample Coagulation Gel - Single-C...
Single-Channel Mechanical Pipettor 1. Product Information Single channel mechanical pipette is ideal tool to support endotoxin detection with Lyophilized Amebocyte Lysate which covers gel-clot technique, kinetic turbidimetric technique, kinetic chromogenic technique, and end-point chromogenic technique. All pipettors are produced by following ISO8655 – 2:2002. The quality control involves gravimetric testing of each pipette with distilled water at 22℃. 2. Product Features: - ...
-
PriceList for Endotoxin Gel Clot Assay – ...
Gel Clot Method Lyophilized Amebocyte Lysate Multi-test Vial G52 series 1. Product Information Gel Clot method Lyophilized Amebocyte Lysate Multi-test Vial is the Lyophilized Amebocyte Lysate reagent which select and use gel clot technique to detect endotoxin or pyrogen. As the widespread method, gel-clot test for endotoxin is simple and does not require specific and expensive instrument. Bioendo provides Gel Clot Lyophilized Amebocyte Lysate reagent in 5.2ml per vial. 2. Product Parameter...
-
2022 China New Design limulus amebocyte lysate ...
Endotoxin-specific Lyophilized Amebocyte Lysate 1. Product Information Lyophilized Amebocyte Lysate extracted from horseshoe crab contains factor C pathway which reacts to endotoxin and factor G pathway which reacts to (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate contains ß-Glucan inhibitor, which will avoid false positive, result happening between factor C pathway and (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate is especially suitable for samples ...
