Wholesale Price Micro kinetic chromogenic endotoxin assay - Endpoint Chromogenic Kit EC80545 – Bioendo
Wholesale Price Micro kinetic chromogenic endotoxin assay - Endpoint Chromogenic Kit EC80545 – Bioendo Detail:
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. Product Information
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis.
2. Product Parameter
Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)
0.1-1.0EU/ml (assay time about 16 minutes)
3. Product Features and Application
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Note:
Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.
|
Catalog No. |
Description |
Kit Contents |
Sensitivity EU/ml |
|
EC80545 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling), 80 Tests/Kit |
5 Lyophilized Amebocyte Lysate, 1.7ml/vial; 4 Water for BET, 50ml/vial; 5 CSE; 5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml /vial; 5 Diazo Reagent 3, 10ml/vial; |
0.1 – 1 EU/ml |
|
EC80545S |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Does the End point endotoxin test kit need sophisticated microplate reader?
Bioendo EC80545 and EC80545S, can read by regular spectrophotometer.
Product detail pictures:
Related Product Guide:
We pursue the administration tenet of "Quality is remarkable, Services is supreme, Status is first", and will sincerely create and share success with all customers for Wholesale Price Micro kinetic chromogenic endotoxin assay - Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Sweden, Jamaica, US, Our solutions are produced with the best raw materials. Every moment, we constantly improve the production programme. In order to ensure better quality and service, we now have been focusing on the production process. We have got high praise by partner. We are looking forward to establishing business relationship with you.
By Elvira from Saudi Arabia - 2018.06.26 19:27
Adhering to the business principle of mutual benefits, we have a happy and successful transaction, we think we will be the best business partner.
By Eartha from Palestine - 2018.02.12 14:52
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