New Fashion Design for Bacterial Endotoxin Test Method Validation Protocol - Pyrogen-free (Endotoxin-free) Tris Buffer – Bioendo
New Fashion Design for Bacterial Endotoxin Test Method Validation Protocol - Pyrogen-free (Endotoxin-free) Tris Buffer – Bioendo Detail:
Pyrogen-free (Endotoxin-free) Tris Buffer
1. Product information
Buffers must be validated to be free of detectable endotoxin andinterfering factors. Using 50mM Tris buffer to dissolve or dilute the testsamples is a convenient way to adjust the reaction pH.
Pyrogen-free (endotoxin-free) Tris buffer to adjusting the pH of the LAL endotoxin test samples.
Lyophilized Amebocyte Lysate testing microbiology detection of endotoxin by horseshoecrab blue blood lysate require certain conditions. The optimal pH for theendotoxin testing Lyophilized Amebocyte Lysate reagent and endotoxin reaction is in the range of 6.0 to8.0. If the endotoxin detection test sample pH is out of this range, the pH maybe adjusted using an acid, base, or endotoxin free suitable buffers. The Acidsand bases may be prepared from concentrates or solids with Lyophilized Amebocyte Lysate Reagent Water incontainers free of detectable endotoxin.
2. Productparameter
Endotoxin level < 0.005EU/ml
3. Productfeatures and application
Adjust the pH of Lyophilized Amebocyte Lysate endotoxin testing in an easy step. Use Tris buffer to dilute the test sample, overcoming the inhibition of Lyophilized Amebocyte Lysate endotoxintesting by adjust the reaction pH to the range of pH 6.0-8.0.
|
Catalog No. |
Description |
Note |
Package |
|
BH10 |
50mM Tris buffer, pH7.0, 10ml/vial |
Used to dilute highly acidic or basic samples. |
10 vials/pack |
|
BH50 |
50mM Tris buffer, pH7.0, 50ml/vial |
Used to dilute highly acidic or basic samples. |
10 vials/pack |
Product Condition
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis, MSDS.
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