High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC64405 – Bioendo
High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC64405 – Bioendo Detail:
End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling)
1. Product Information
End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is conducted by adding a colorless artificial peptide substrate solution into mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. If test sample contain endotoxin, a yellow color will develop in the 96 well microplate. Its absorbance (λmax = 405nm) is related to the endotoxin concentration. Endotoxin concentration of test sample could be calculated based on a standard curve.
2. Product parameter
Sensitivity Range: 0.01-0.1 EU/ml, matching assay time is about 46 minutes
0.1-1 EU/ml, matching assay time is about 16 minutes.
3. Product Features and Application
End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into the mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. Yellow color develops if test sample contains endotoxin. Then use regular spectrophotometer or plate reader to read the absorbance at 405nm. End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is suitable for all kinds of biomedical samples such as protein, vaccine, plasmid, DNA, RNA endotoxin assay.
Note:
Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.
|
Catalog No. |
Description |
Kit Contents |
Sensitivity (EU/ml) |
|
EC64405 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay), 64 Tests/Kit
|
2 Lyophilized Amebocyte Lysate, 1.7ml/vial; 2 Water for BET, 50ml/vial; 2 CSE; 4 Chromogenic Substrate, 1.7ml/vial; |
0.1 – 1 EU/ml |
|
EC64405S |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/m |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Does the End point endotoxin test kit need sophisticated microplate reader?
Bioendo EC64405 and EC64405S kit analysis the quantitative endotoxin results through regular microplate reader.
Endotoxin Free Tube
Pyrogen Free Tips
Pyrogen Free Microplates
Regular Microplate reader
Matched Control standard endotoxin (CSE10V)
BET water use for reconstitution.
Product detail pictures:
Related Product Guide:
The key to our success is "Good Product Quality, Reasonable Value and Efficient Service" for High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC64405 – Bioendo , The product will supply to all over the world, such as: Grenada, Anguilla, venezuela, we've got all day online sales to make sure the pre-sale and after-sale service in time. With all these supports, we can serve every customer with quality product and timely shipping with highly responsibility. Being a young growing company, we might not the best, but we are trying our best to be your good partner.
By Eunice from Slovenia - 2018.09.23 18:44
Managers are visionary, they have the idea of "mutual benefits, continuous improvement and innovation", we have a pleasant conversation and Cooperation.
By Flora from New Orleans - 2018.05.15 10:52
Leave Your Messages
Related products
-
Wholesale Pyrogen free tip - Vortex Mixer R...
Vortex mixer 1. Product information The Vortex mixer widely used in life science, physical and chemicalanalysis fields, for vigorous re-suspension of cells or chemical pelletsin tubes up to 15 ml. It is good for the dilution and mixing the Control Standard Endotoxin solution in endotoxin assay with gel clot method, kinetic chromogenic method, kinetic turbidimetric method and end-point chromogenic method. 2. Features - Touch and continuous operations - with wide speed range of 0-2500rpm,st...
-
Manufacturer for Chromogenic endotoxin detectio...
Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) 1. Product Information In Bioendo KC Endotoxin Test Kit, Amebocyte Lysate is co-lyophilized with chromogenic substrate. Therefore, bacterial endotoxin could be quantified based on the chromogenic reaction. The assay is strong resistance to interference, and has advantages of kinetic turbidimetric and end-point chromogenic method. Bioendo Endotoxin Test Kit contains Chromogenic Amebocyte Lysate, Reconstitution Buffer, CSE, Water for BE...
-
Factory Cheap Hot limulus amebocyte lysate kit ...
Endotoxin-specific Lyophilized Amebocyte Lysate 1. Product Information Lyophilized Amebocyte Lysate extracted from horseshoe crab contains factor C pathway which reacts to endotoxin and factor G pathway which reacts to (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate contains ß-Glucan inhibitor, which will avoid false positive, result happening between factor C pathway and (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate is especially suitable for samples ...
-
Factory wholesale Amebocyte Lysate - Endotoxin...
Endotoxin-specific Lyophilized Amebocyte Lysate 1. Product Information Lyophilized Amebocyte Lysate extracted from horseshoe crab contains factor C pathway which reacts to endotoxin and factor G pathway which reacts to (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate contains ß-Glucan inhibitor, which will avoid false positive, result happening between factor C pathway and (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate is especially suitable for samples ...
-
OEM manufacturer Endotoxin Elisa Kit - Control...
Control Standard Endotoxin (CSE) 1. Product Information Control Standard Endotoxin (CSE) is extracted from E.coli O111:B4. CSE is an economic alternative to Reference Standard Endotoxin (RSE) in constructing standard curves, validating product and preparing controls in Lyophilized Amebocyte Lysate test. The labeled potency of CSE endotoxinE.coli standard is referenced against RSE. The Control Standard Endotoxin could be used with gel clot assay, kinetic turbidimetric assay or kinetic chromog...
-
Cheap price Endotoxin free pipette tips - Beta...
Beta-glucans Blocker for Blocking Beta Glucan Pathway 1. Product information There are two pathway in limulus amoebocyte lysate lal reagent, factor C pathway is specific to endotoxin and factor G pathway is specific to(1,3)- β-D-Glucans. If the test sample contains β-1,3-Glucans, the limulus test (Endotoxin test ) will have interferences. β-G-Blocker blocks the reactivity of LAL to β-1,3-Glucans，conferring increased endotoxin specificity to the LAL test. If the test samples containsβ-1,3-Gl...
