Factory Cheap Hot LAL chromogenic detection - Endpoint Chromogenic Kit EC80545 – Bioendo
Factory Cheap Hot LAL chromogenic detection - Endpoint Chromogenic Kit EC80545 – Bioendo Detail:
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. Product Information
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis.
2. Product Parameter
Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)
0.1-1.0EU/ml (assay time about 16 minutes)
3. Product Features and Application
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Note:
Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.
|
Catalog No. |
Description |
Kit Contents |
Sensitivity EU/ml |
|
EC80545 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling), 80 Tests/Kit |
5 Lyophilized Amebocyte Lysate, 1.7ml/vial; 4 Water for BET, 50ml/vial; 5 CSE; 5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml /vial; 5 Diazo Reagent 3, 10ml/vial; |
0.1 – 1 EU/ml |
|
EC80545S |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Does the End point endotoxin test kit need sophisticated microplate reader?
Bioendo EC80545 and EC80545S, can read by regular spectrophotometer.
Product detail pictures:
Related Product Guide:
We depend on sturdy technical force and continually create sophisticated technologies to meet the demand of Factory Cheap Hot LAL chromogenic detection - Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Venezuela, Toronto, Puerto Rico, Our product quality is one of the major concerns and has been produced to meet the customer's standards. "Customer services and relationship" is another important area which we understand good communication and relationships with our customers is the most significant power to run it as a long term business.
By Meredith from Macedonia - 2018.04.25 16:46
Good quality and fast delivery, it's very nice. Some products have a little bit problem, but the supplier replaced timely, overall, we are satisfied.
By Arabela from Singapore - 2017.09.09 10:18
Leave Your Messages
Related products
-
High definition Pipette tip - Mini Dry Heat In...
Dry heat incubator single module 1. Product information The Mini Dry Heat Incubator is a micro-processor controlled heating block with semi conductor heating technology.It adapts onboard use, smart, light and convenient for movement, suit for any kind of occasions. Especially good for the incubation of the gel clot LAL assay, LAL chromogenic endpoint assay incubation. 2. Product features 1. Unique designed. Smart and light, convenient movement, suit for various occasions. 2. LCD simultaneou...
-
Hot sale Kinetic Turbidimetric TAL Assay - Kin...
Kinetic Turbidimetric Amebocyte Lysate Vial 1. Product Introduction Kinetic Turbidimetric Amebocyte Lysate Vial is developed based on the principle that the time needed to reach a certain absorbance increase (onset OD), i.e. onset time, is negatively correlated with the endotoxin concentration. Sensitivity could reach 0.005EU/ml, and the detection could reach four orders of magnitude. It is specially suitable for pharmaceuticals industry to monitor endotoxin concentration. 2. Product Parame...
-
Best quality limulus lysate assay - Endotoxin-...
Endotoxin-specific Lyophilized Amebocyte Lysate 1. Product Information Lyophilized Amebocyte Lysate extracted from horseshoe crab contains factor C pathway which reacts to endotoxin and factor G pathway which reacts to (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate contains ß-Glucan inhibitor, which will avoid false positive, result happening between factor C pathway and (1,3)- ß-D-Glucan. Endotoxin-specific Lyophilized Amebocyte Lysate is especially suitable for samples ...
-
Excellent quality Toxinsensor Chromogenic LAL E...
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) 1. Product Information Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum abso...
-
Factory directly Endotoxin Free Test Tubes - E...
Endotoxin Challenge Vials (Endotoxin Indicator) 1. Product Information The Endotoxin Challenge Vial (ECV，Endotoxin Indicator) is used in the validation of dry heat depyrogenation cycles. Endotoxin Challenge Vials are placed in the cold spots of the dry heat oven. After cycle completion, the log reduction in endotoxin levels can be determined by comparing the endotoxin levels in the baked vs not baked Endotoxin Indicators. The Endotoxin Challenge vials are designed to indicate a minimum 3...
-
OEM/ODM Manufacturer Endotoksin Testi - Rapid ...
Rapid Gel Clot Endotoxin Assay Kit (10 Samples Kit) 1. Product Information Bioendo Rapid Gel Clot Endotoxin Assay Kit is designed to rapidly quantify endotoxin in water or dialysate. Generally, RG kit’s result could be gained within 30 minutes. Under the guidance of detecting endotoxin in water or dialysate quickly, endotoxin detection with Bioendo Rapid Gel Clot Endotoxin Assay Kit does not need the multi steps’ dilution of Control Standard Endotoxin and test samples. Operation procedure...
