Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo
Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo Detail:
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. Product Information
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis.
2. Product Parameter
Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)
0.1-1.0EU/ml (assay time about 16 minutes)
3. Product Features and Application
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Note:
Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.
|
Catalog No. |
Description |
Kit Contents |
Sensitivity EU/ml |
|
EC80545 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling), 80 Tests/Kit |
5 Lyophilized Amebocyte Lysate, 1.7ml/vial; 4 Water for BET, 50ml/vial; 5 CSE; 5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml /vial; 5 Diazo Reagent 3, 10ml/vial; |
0.1 – 1 EU/ml |
|
EC80545S |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Does the End point endotoxin test kit need sophisticated microplate reader?
Bioendo EC80545 and EC80545S, can read by regular spectrophotometer.
Product detail pictures:
Related Product Guide:
To be a result of ours specialty and service consciousness, our company has won a superb reputation between customers all around the environment for Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Denver, Surabaya, Switzerland, Our staffs are rich in experience and trained strictly, with qualified knowledge, with energy and always respect their customers as the No. 1, and promise to do their best to deliver the effective and individual service for customers. The Company pays attention to maintaining and developing the long-term cooperation relationship with the customers. We promise, as your ideal partner, we'll develop a bright future and enjoy the satisfying fruit together with you, with persisting zeal, endless energy and forward spirit.
By Fay from South Africa - 2017.06.29 18:55
In China, we have purchased many times, this time is the most successful and most satisfactory, a sincere and realiable Chinese manufacturer!
By Darlene from Estonia - 2018.04.25 16:46
Leave Your Messages
Related products
-
Good quality Turbidimetric LAL test - Kinetic ...
Kinetic Turbidimetric Amebocyte Lysate Vial 1. Product Introduction Kinetic Turbidimetric Amebocyte Lysate Vial is developed based on the principle that the time needed to reach a certain absorbance increase (onset OD), i.e. onset time, is negatively correlated with the endotoxin concentration. Sensitivity could reach 0.005EU/ml, and the detection could reach four orders of magnitude. It is specially suitable for pharmaceuticals industry to monitor endotoxin concentration. 2. Product Parame...
-
Wholesale Price China LAL bacterial endotoxin t...
Endotoxin-free Glass Test Tubes (Endotoxin free tubes) 1. Product Information Endotoxin-free glass test tubes contain less than 0.005EU/ml endotoxin. Catalog number T107505 and T107540 are recommended for use as reaction tubes in gel clot and end-point chromogenic assays. Catalog number T1310018 and T1310005 is recommended for dilution of endotoxin standards and test samples. T1050005C is a special designed short endotoxin reaction tube that allows the pipette tips reaches the tube bottom. ...
-
Factory Cheap Hot Bacterial Endotoxin Test Gel ...
Gel Clot Lyophilized Amebocyte Lysate Single Test in Vial 1. Product Information Gel Clot Lyophilized Amebocyte Lysate Single Test in Vial Contain endotoxin-specific Amebocyte Lysate which includes beta-glucan inhibitor in the formulation and will not react to beta-glucan. For our Single Test in Vial, you could add sample to the vial directly. This means you don’t need to reconstitute Amebocyte Lysate at first, and you will decide how many vials use each time to avoid waste. Endotoxin free t...
-
2022 wholesale price Kinetic Turbidimetric vs K...
Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) 1. Product Information In Bioendo KC Endotoxin Test Kit, Amebocyte Lysate is co-lyophilized with chromogenic substrate. Therefore, bacterial endotoxin could be quantified based on the chromogenic reaction. The assay is strong resistance to interference, and has advantages of kinetic turbidimetric and end-point chromogenic method. Bioendo Endotoxin Test Kit contains Chromogenic Amebocyte Lysate, Reconstitution Buffer, CSE, Water for BE...
-
High definition LAL Turbidimetric detection - ...
Bioendo KT Endotoxin Test Kit (Kinetic Turbidimetric Assay) 1. Product Introduction Kinetic Turbidimetric Amebocyte Lysate Vial is developed based on the principle that the time needed to reach a certain absorbance increase (onset OD), i.e. onset time, is negatively correlated with the endotoxin concentration. Sensitivity could reach 0.005EU/ml, and the detection could reach four orders of magnitude. It is specially suitable for pharmaceuticals industry to monitor endotoxin concentration. T...
-
Chinese wholesale Kinetic chromogenic TAL Assay...
End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) 1. Product Information End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is conducted by adding a colorless artificial peptide substrate solution into mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. If test sample contain endotoxin, a yellow color will develop in the 96 well microplate. Its absorbance (λmax = 405nm) is related to the endotoxin concentration. Endotoxin ...
