2022 Good Quality Kinetic chromogenic TAL test - Endpoint Chromogenic Kit EC80545 – Bioendo
2022 Good Quality Kinetic chromogenic TAL test - Endpoint Chromogenic Kit EC80545 – Bioendo Detail:
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. Product Information
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis.
2. Product Parameter
Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)
0.1-1.0EU/ml (assay time about 16 minutes)
3. Product Features and Application
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Note:
Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.
|
Catalog No. |
Description |
Kit Contents |
Sensitivity EU/ml |
|
EC80545 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling), 80 Tests/Kit |
5 Lyophilized Amebocyte Lysate, 1.7ml/vial; 4 Water for BET, 50ml/vial; 5 CSE; 5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml /vial; 5 Diazo Reagent 3, 10ml/vial; |
0.1 – 1 EU/ml |
|
EC80545S |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Does the End point endotoxin test kit need sophisticated microplate reader?
Bioendo EC80545 and EC80545S, can read by regular spectrophotometer.
Product detail pictures:
Related Product Guide:
We attempt for excellence, company the customers", hopes to be the top cooperation team and dominator company for personnel, suppliers and customers, realizes price share and continual marketing for 2022 Good Quality Kinetic chromogenic TAL test - Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Greek, Uruguay, Lesotho, We warmly welcome domestic and overseas customers to visit our company and have business talk. Our company always insists on the principle of "good quality, reasonable price, the first-class service". We are willing to build long-term, friendly and mutually beneficial cooperation with you.
By Arlene from Portugal - 2017.06.25 12:48
This is the first business after our company establish, products and services are very satisfying, we have a good start, we hope to cooperate continuous in the future!
By Marcia from Portland - 2017.11.01 17:04
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